Journal: BMC Oral Health

Article Title: Edaravone attenuates doxorubicin-induced oral mucosal injury via modulation of oxidative stress, inflammatory signaling, and the SIRT1/TLR4/NF-kB/ACE2 axis in rats

doi: 10.1186/s12903-025-07148-y

Figure Lengend Snippet: Tongue tissue TLR4 and proinflammatory cytokines. A TLR4 (ng/mg protein), ( B ) TNF-α (ng/L/g), ( C ) IL-6 (ng/L/g) (mean ± SEM). DOX increased TLR4/TNF-α/IL-6; EDO reduced these levels. Statistics: TLR4/IL-6—ANOVA ± Tukey; TNF-α—Kruskal–Wallis ± Mann–Whitney. Significance: * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant (adjusted p ≥ 0.05)

Article Snippet: TLR4 protein levels in tongue tissue were quantified using a rat TLR4 ELISA kit (Cusabio, Cat. No: CSB-E15822r).

Techniques: MANN-WHITNEY

Journal: Pharmaceuticals

Article Title: Gum Acacia–Dexamethasone Combination Attenuates Sepsis-Induced Acute Kidney Injury in Rats via Targeting SIRT1-HMGB1 Signaling Pathway and Preserving Mitochondrial Integrity

doi: 10.3390/ph18081164

Figure Lengend Snippet: Impact of GA, DEX, and combination of GA and DEX on LPS-induced changes in inflammatory markers. ( A ) Renal SIRT1 level, ( B ) renal TLR4 level, ( C ) renal MYD88 level, ( D ) renal IL-1β level, and ( E ) renal TNF-α level. LPS: lipopolysaccharide; DEX: dexamethasone; GA: Gum acacia; SIRT1: Silent information regulator 2-related enzyme 1; TLR4: Toll-like receptor 4; TNF-α: tumor necrosis factor α; IL-1β: interleukin -1β; MYD88: myeloid differentiation primary response 88. GA administration started on day 1 and lasted for 14 days. LPS was injected on day 14, and DEX was injected 2 h post LPS injection. Data were expressed as means ± S.E.M. (n = 6 rats per group). Mean values were compared using one-way ANOVA followed by post hoc Tukey’s multiple comparison test: * p < 0.05, vs. CTR group; # p < 0.05, vs. LPS group.

Article Snippet: Inflammation Biomarkers: Sirtuin-1 (SIRT1) (BT LABORATORY, Beijing, China, E1145Ra), tumor necrosis factor α (TNF-α) (CUSABIO, Houston, TA, USA, CSB-E11987r), Toll-like receptor 4 (TLR4) (CUSABIO, Houston, TA, USA, CSB-E15822r), myeloid differentiation primary response 88 (MYD88) (Assay Genie, Dublin, Ireland, RTFI01303), and interleukin-1β (IL-1β) (CUSABIO, Houston, TA, USA, CSB-E08055r) were quantified in kidney homogenate using ELISA kits according to the manufacturer’s instructions.

Techniques: Injection, Comparison

Journal: Microbial pathogenesis

Article Title: Exploring the effects of lactulose on lung-intestinal tissue-associated factors and the TLR4/NF-κB signaling pathway in COPD rats based on lung-gut axis theory.

doi: 10.1016/j.micpath.2024.107245

Figure Lengend Snippet: Fig. 6. Protein band diagrams of MUC5AC, TLR4, and p-P65 in lung tissue among different rat groups.

Article Snippet: LPS and 2 % pentobarbital were both sourced from Beijing Ita Biotechnology Co., Ltd., with batch numbers YT1319 and 23NO213, respectively; Hardman brand cigarettes were provided by Shandong Zhongshan Cigarette Industry Co., Ltd., with tar content of 10 mg, nicotine content of 0.8 mg, and carbon monoxide content of 13 mg; phosphate buffer saline (PBS) buffer was supplied by Wuxi Aorui Dongyuan Biotechnology Co., Ltd., with lot number 21031203; 2.5 % glutaraldehyde was from Beijing Solepol Science and Technology Co., Ltd., with lot number P1126; anhydrous ethanol and acetone were both from Sinopharm Chemical Reagent Co., Ltd., with lot numbers 100092183 and 10000418, respectively; 812 embedding agent was sourced from SPI, with batch number 90529-77-4; eosin staining solution and hematoxylin staining solution were both from Zhuhai Beso Biotechnology Co. Ltd., with batch numbers BA4024 and BA4041, respectively; TNF-α ELISA kit and IL-1β ELISA kit were provided by Jiangsu Zeyu Bio-technology Co. Ltd., with batch numbers 2312226006R and 231226017R, respectively; reverse transcription kit was supplied by Thermo, USA, with lot number K1622; rabbit anti-rat mucin 5AC (MUC5AC) antibody was sourced from Invitrogen, with lot number PA5-115601; rabbit anti-rat p65 phosphorylated antibody, rabbit anti-rat p65 antibody, and mouse anti-rat TLR4 antibody were all provided by Proteintech, with batch numbers 82335-1-RR, 10745-1-AP, and 66350-1-Ig, respectively; goat anti-rabbit IgG antibody was bought from Affinity, with batch number S0001; and sheep anti-mouse IgG antibody was sourced from Affinity, with batch number S0002.

Techniques:

Journal: Microbial pathogenesis

Article Title: Exploring the effects of lactulose on lung-intestinal tissue-associated factors and the TLR4/NF-κB signaling pathway in COPD rats based on lung-gut axis theory.

doi: 10.1016/j.micpath.2024.107245

Figure Lengend Snippet: Fig. 8. Protein band diagrams of MUC2, TLR4, and p-P65 in colon tissue among different rat groups.

Article Snippet: LPS and 2 % pentobarbital were both sourced from Beijing Ita Biotechnology Co., Ltd., with batch numbers YT1319 and 23NO213, respectively; Hardman brand cigarettes were provided by Shandong Zhongshan Cigarette Industry Co., Ltd., with tar content of 10 mg, nicotine content of 0.8 mg, and carbon monoxide content of 13 mg; phosphate buffer saline (PBS) buffer was supplied by Wuxi Aorui Dongyuan Biotechnology Co., Ltd., with lot number 21031203; 2.5 % glutaraldehyde was from Beijing Solepol Science and Technology Co., Ltd., with lot number P1126; anhydrous ethanol and acetone were both from Sinopharm Chemical Reagent Co., Ltd., with lot numbers 100092183 and 10000418, respectively; 812 embedding agent was sourced from SPI, with batch number 90529-77-4; eosin staining solution and hematoxylin staining solution were both from Zhuhai Beso Biotechnology Co. Ltd., with batch numbers BA4024 and BA4041, respectively; TNF-α ELISA kit and IL-1β ELISA kit were provided by Jiangsu Zeyu Bio-technology Co. Ltd., with batch numbers 2312226006R and 231226017R, respectively; reverse transcription kit was supplied by Thermo, USA, with lot number K1622; rabbit anti-rat mucin 5AC (MUC5AC) antibody was sourced from Invitrogen, with lot number PA5-115601; rabbit anti-rat p65 phosphorylated antibody, rabbit anti-rat p65 antibody, and mouse anti-rat TLR4 antibody were all provided by Proteintech, with batch numbers 82335-1-RR, 10745-1-AP, and 66350-1-Ig, respectively; goat anti-rabbit IgG antibody was bought from Affinity, with batch number S0001; and sheep anti-mouse IgG antibody was sourced from Affinity, with batch number S0002.

Techniques:

Journal: Microbial pathogenesis

Article Title: Exploring the effects of lactulose on lung-intestinal tissue-associated factors and the TLR4/NF-κB signaling pathway in COPD rats based on lung-gut axis theory.

doi: 10.1016/j.micpath.2024.107245

Figure Lengend Snippet: Fig. 6. Protein band diagrams of MUC5AC, TLR4, and p-P65 in lung tissue among different rat groups.

Article Snippet: LPS and 2 % pentobarbital were both sourced from Beijing Ita Biotechnology Co., Ltd., with batch numbers YT1319 and 23NO213, respectively; Hardman brand cigarettes were provided by Shandong Zhongshan Cigarette Industry Co., Ltd., with tar content of 10 mg, nicotine content of 0.8 mg, and carbon monoxide content of 13 mg; phosphate buffer saline (PBS) buffer was supplied by Wuxi Aorui Dongyuan Biotechnology Co., Ltd., with lot number 21031203; 2.5 % glutaraldehyde was from Beijing Solepol Science and Technology Co., Ltd., with lot number P1126; anhydrous ethanol and acetone were both from Sinopharm Chemical Reagent Co., Ltd., with lot numbers 100092183 and 10000418, respectively; 812 embedding agent was sourced from SPI, with batch number 90529-77-4; eosin staining solution and hematoxylin staining solution were both from Zhuhai Beso Biotechnology Co. Ltd., with batch numbers BA4024 and BA4041, respectively; TNF-α ELISA kit and IL-1β ELISA kit were provided by Jiangsu Zeyu Bio-technology Co. Ltd., with batch numbers 2312226006R and 231226017R, respectively; reverse transcription kit was supplied by Thermo, USA, with lot number K1622; rabbit anti-rat mucin 5AC (MUC5AC) antibody was sourced from Invitrogen, with lot number PA5-115601; rabbit anti-rat p65 phosphorylated antibody, rabbit anti-rat p65 antibody, and mouse anti-rat TLR4 antibody were all provided by Proteintech, with batch numbers 82335-1-RR, 10745-1-AP, and 66350-1-Ig, respectively; goat anti-rabbit IgG antibody was bought from Affinity, with batch number S0001; and sheep anti-mouse IgG antibody was sourced from Affinity, with batch number S0002.

Techniques:

Journal: Microbial pathogenesis

Article Title: Exploring the effects of lactulose on lung-intestinal tissue-associated factors and the TLR4/NF-κB signaling pathway in COPD rats based on lung-gut axis theory.

doi: 10.1016/j.micpath.2024.107245

Figure Lengend Snippet: Fig. 8. Protein band diagrams of MUC2, TLR4, and p-P65 in colon tissue among different rat groups.

Article Snippet: LPS and 2 % pentobarbital were both sourced from Beijing Ita Biotechnology Co., Ltd., with batch numbers YT1319 and 23NO213, respectively; Hardman brand cigarettes were provided by Shandong Zhongshan Cigarette Industry Co., Ltd., with tar content of 10 mg, nicotine content of 0.8 mg, and carbon monoxide content of 13 mg; phosphate buffer saline (PBS) buffer was supplied by Wuxi Aorui Dongyuan Biotechnology Co., Ltd., with lot number 21031203; 2.5 % glutaraldehyde was from Beijing Solepol Science and Technology Co., Ltd., with lot number P1126; anhydrous ethanol and acetone were both from Sinopharm Chemical Reagent Co., Ltd., with lot numbers 100092183 and 10000418, respectively; 812 embedding agent was sourced from SPI, with batch number 90529-77-4; eosin staining solution and hematoxylin staining solution were both from Zhuhai Beso Biotechnology Co. Ltd., with batch numbers BA4024 and BA4041, respectively; TNF-α ELISA kit and IL-1β ELISA kit were provided by Jiangsu Zeyu Bio-technology Co. Ltd., with batch numbers 2312226006R and 231226017R, respectively; reverse transcription kit was supplied by Thermo, USA, with lot number K1622; rabbit anti-rat mucin 5AC (MUC5AC) antibody was sourced from Invitrogen, with lot number PA5-115601; rabbit anti-rat p65 phosphorylated antibody, rabbit anti-rat p65 antibody, and mouse anti-rat TLR4 antibody were all provided by Proteintech, with batch numbers 82335-1-RR, 10745-1-AP, and 66350-1-Ig, respectively; goat anti-rabbit IgG antibody was bought from Affinity, with batch number S0001; and sheep anti-mouse IgG antibody was sourced from Affinity, with batch number S0002.

Techniques: